Comparative antibacterial studies of in vivo and in vitro leaves of Lawsonia inermis L.- A multipurpose medicinal plant

Plant Science Research 35 (1&2) : 47-50, 2014

A. Moharana1, S. Kumar1, P. K. Jena1, S. K. Naik1, S. Bal2 and D. P. Barik1Ψ
1 Department of Botany, Ravenshaw University, Cuttack-753 003, Odisha, India
2 Directroate of Oil Seed Research, Hyderabad-500 030, TG, India

Abstract

A comparative antibacterial study of in vivo and in vitro leaf extracts was performed against four different bacterial strains viz., Streptococcus pyogenes, Streptococcus mutans, Shigella flexnerii and Salmonella enteric-typhii. Both types of leaf materials (in vivo and in vitro) of L. inermis screened for four different solvent systems, showed anti-bacterial activity (MIC values) against all human pathogenic bacteria tested. Of the four bacterial strains evaluated, the best MIC value (200 μg/ml) was observed in in vitro leaf extract of all the solvent system against Streptococcus pyogenes. Acetone and n-hexane in vitro leaf extract was found to be more effective (MIC = 200 μg/ml) than in vivo leaf extract (MIC = 400 μg/ml) for S. pyogenes and S. mutans, respectively. The results of this study tend to give credence to the use of henna leaf of in vitro regenerated plants as antibacterial agent in pharmaceutical industries without disturbing the garden plants.

Natural dye-yielding plants of tribal Odisha



Plant Lovers' Association, Bhubaneswar

Sabujima, 23: 23-25


Natural dye-yielding plants of tribal Odisha

Sanjeet Kumar and Padan Kumar Jena

Department of Botany, Ravenshaw University, Cuttack-753003, Odisha
sanjeet.biotech@gmail.com

Color is of paramount importance in nature that makes human living more aesthetic and fascinating. It is supposed to be associated with seasons, festivals, human emotions, qualities and passion of life. A dye gives a color. A dye can generally be described as a coloured substance that has an affinity to the substrate to which it is applied. A dye, which is resistance to light, water and soap. There are two types of dye, natural and synthetics. Natural dyes are dyes or colorants derived from plants, animals, minerals or other natural resources whereas synthetic dyes are synthesised from different chemicals. The majority of natural dyes are obtained from plant parts, such as leaves, flowers, rhizomes, tubers, fruits, bark and seeds.
The art of dyeing is as old as human civilization. From the historical records, it is learnt that natural colorants were available to people during Greco-Roman periods and Neolithic periods. Dyeing with plant parts has been traced back to more than 5,000 years in China. Ancient Egyptian hieroglyphs contain detail description of the extraction of natural dyes and their application in dyeing. Dyes such as Cochineal and Logwood were brought to Europe by the Spanish treasure fleets, and the dyestuffs of Europe were carried by colonists to America (Barber, 1991). An anonymous researcher has reported that an orange or yellow impermanent dye is made from flowers of Nyctanthes arbor-tristis used for making Buddhist robes in Sri Lanka. The use of natural dyeing materials is evident with the wall paintings of Ajanta, Ellora and Sithannvasal in India. They still demonstrate the efficacy of dyeing craft that had been inherited from ancient times. The knowledge of dyeing was also known to people of Indus Valley Civilisation. This has been substantiated by the findings of coloured garments and traces of madder (Rubia cordifolia) dye in the ruins of the Indus Valley Civilisation at Mohenjo-Daro and Harappa.  Traditionally ‘Holi’ was also played with flower petals or with natural colors obtained from different plant parts. Simili and Palasha were widely used for this purpose.
Although dye might have discovered accidentally, but the art of dyeing spread widely as civilisation advanced and today, dyeing has become a complex and specialized science. The increasing market demand for dyes and the dwindling numbers of dye yielding plants forced the emergence of synthetic dyes like aniline, rhodamine, mayhem’s dye etc., which have threatened the total replacement of natural dyes. However, even today, efforts are being made to derive dyes from natural resources. Dye for eye shadows is obtained from Indigo, while a dye for lipstick is still obtained from seed of annato. Yellow dye from turmeric, not only is a dye but a powerful antiseptic that revitalizes the skin. Indigo dye yields a cooling sensation like sandal wood in addition to its colouring effect. Synthetic dyes are suspected to release harmful chemicals that are allergic, carcinogenic and detrimental to human health. Therefore, there is a need for screening and documentation of plant species having dyeing properties. Literature survey has shown that studies are continuously being made to identify dye yielding plants and dyes derived from them throughout the globe. However dye yielding plants have not been properly documented in Odisha. Hence, a study was made to document such plants that are used to yield dye by the tribal communities of Odisha.
Our state Odisha is situated between 17° 49´ to 22° 34´ North latitude and 81° 28´ to 87° 29´ East longitude. The state of West Bengal lies towards its North-East, Jharkhand towards North, Chhattisgarh towards West, Andhra Pradesh towards South and the Bay of Bengal towards East. It has an area of 155707 sq. Kms., which comprises 4.74 % of country’s land mass.  Physio-graphically, Odisha is not homogeneous due to its mountainous terrain, major rivers and their tributaries. It has limited area of lower elevation while about three-fourth of its land surface is hilly and mountainous. State claims to have a prominent position among the states and Union Territories of the country for having the largest ethnic groups (62) including 13 vulnerable tribal communities. It is having the third highest tribal population numbering to about 8 millions, which is about 9.7 % of the country’s total population and 22.13 % of the state’s total population.
Each ethnic tribe possesses its distinct identity as to their language, culture, festival, rituals, religion, socio-cultural features, demographic characteristics and their dependence on natural resources. They collect the leaves, fruits, flowers, roots, tubers, nuts, bark and whole plants for their day to day use.  For painting and dyeing, they use the many plant parts. Our knowledge on dyeing with natural resources in mainly based on the tribal use of natural dye. They have unique skill to isolate the crude dye from floral wealth. At times they use dye for colouring their food too. Many of these dyes used by them are eco-friendly and non-toxic. Present study deals with, 43 plant species belonging to 27 families and 41 genus used as natural dye by the tribal of Odisha.  Among the documented plants, flowers are more frequently used to make dye followed by bark and fruits and the whole plant in least used as source of dye (Fig-1; Table-1).
Table: Most common Dye yielding plants of Odisha
Plant Name
Local name
Family
Parts used
Colour obtained
Abrus precatorius
Kāincho
Fabaceae
Seeds
Black
Acacia catechu
Khairo
Mimosaceae
Bark
Pink
Acacia nilotica
Babul
Mimosaceae
Gum
Black
Aegle marmelos
Bela
Rutaceae
Fruits
Yellow
Barleria prionitis
Kanta Jati
Acanthaceae
Flowers
Yellow
Basella alba
Poi
Basellaceae
Fruits
Blue
Bauhinia purpurea
Kuilari
Bark
Violet
Bixa orellana
Sundry
Bixaceae
Seeds
Red/ Orange red
Bombax ceiba
Similli
Bombacaceae
Flowers
Red
Butea monosperma
Palasa
Fabaceae
Flower
Yellow / Orange
Butea superba
Lata Palasa
Fabaceae
Flower
Deep yellow
Cassia fistula
Sunari
Bark
Brown
Cassytha filiformis
Nirmuli
Lauraceae
Stem
Brown
Catharanthus roseus
Sadabihari
Apocynaceae
Flowers
Pink / Red
Clitoria ternatea
Aprajita
Fabaceae
Flower
Blue
Curcuma longa
Haldi
Zingiberaceae
Rhizomes
Yellow
Dioscorea bulbifera
Pita Aalu
Dioscoreaceae
Tuber
Pale yellow
Enhydra fluctuans
Hidmichia
Asteraceae
Leaves
Green
Erythrina suberosa
Paldhua
Fabaceae
Flowers
Pink/ Orange
Indigofera tinctoria
Nilli
Fabaceae
Flowers
Indigo / Blue
Lannea coromandelica
Jia
Anacardiaceae
Bark
Red
Lawsonia inermis
Manjuati
Lythraceae
Leaves
Deep red / Orange
Madhuca longifolia
Mahula
Sapotaceae
Flowers
Red
Mallotus philippensis
Basanto Gundi
Euphorbiaceae
Fruits
Red
Melastoma malabathricum
Karati
Melastomataceae
Fruits
Purple
Michelia champaca
Champa
Magnoliaceae
Flower
Yellow
Mirabilis jalapa
Rangani
Nyctaginaceae
Flowers
Pinkish red
Nyctanthes arbor-tristis
Gangasuali
Oleaceae
Flower
Orange
Nymphaea pubescens
Nalikain
Nymphaeaceae
Rhizome
Blue
Oldenlandia umbellata
Subulli
Rubiaceae
Root
Red
Pithecellobium dulce
Sima Kaian
Mimosaceae
Bark
Pink
Polygonum hydropiper
Bihagani
Polygonaceae
Leaves
Blue
Pterocarpus marsupium
Piasal
Fabaceae
Bark
Red
Pterocarpus santalinus
Rakta Chandan
Fabaceae
Bark
Red
Punica  granatum
Dalimba
Lythraceae
Fruits
Yellow
Rubia cordifolia
Rango Chero
Rubiaceae
Whole plant
Deep red
Semecarpus anacardium
Bhalia
Anacradiaceae
Fruits
Black
Syzygium cumini
Jamu
Myrtaceae
Fruits
Blue
Tagetes erecta
Gendu
Asteraceae
Flowers
Yellow
Tagetes patula
Gendu
Asteraceae
Flowers
Reddish yellow
Tectona grandis
Tiko
Verbenaceae
Leaves
Brick red
Terminalia chebula
Harida
Combrataceae
Bark
Blackish brown
Woodfordia fruticosa
Dhataki
Lythraceae
Flowers
Red

The study indicates that Odisha is endowed with wealth of natural flora that provides the basic resources for rainbow of natural dyes. Natural dyes are less toxic, non-carcinogenic and less polluting in comparison to synthetic dyes. People of the state are in habit of using synthetic dyes to carter their need as they are easily available in the market on festive occasions either to color the mother earth or to color the statue of God & Goddess, we use synthetic dyes. After the emersion of these statues in the river or in other water bodies, the water gets polluted. This creates a major pollution problem after the festive occasions are over. The easiest solution to such problems is to use dyes derived from natural sources that are non-toxic and non-polluting.
Although tribal Odisha have enough resource of dye yielding plants, but very less of them have been exploited. Due to lack of technical knowledge, coupled with reluctance of young generations towards this tedious task has ultimately resulted in ignorance of these natural wealth. Detail studies and scientific investigations are needed to assess the real potential and availability of natural dye yielding resources in tribal Odisha. As lack of awareness and conservation strategy might lead to depletion of many of these natural resources. It is the right time to document these natural treasures and work on them for their sustainability. Such study will not only help in conservation of phyto-resources of the state but also will add to the progressive development of tribal knowledge available in the state.


Floral wealth of Mahanadi River