DOWN STREAM PROCESSING
Sanjeet Kumar Ravenshaw University sanjeet.biotech@gmail.com · Downstream processing refers to the recovery and purification of biosynthetic products. The extraction and purification of a biotechnological product from fermentation is referred to as downstream processing (DSP) or product recovery. · DSP is as complex and important as fermentation process. · The methodology adopted for downstream processing depends on the nature of the end product, its concentration, stability and the degree of purification required, besides on the presence of other products. · The desired products for isolation by DSP are most frequently metabolites which may be present as follows:- 1. Intracellular metabolites- Vitamins 2. Extracellular metabolites- amino acids 3. Both Intra- and Extra- cellular- Flavomycin Stages in Downstream processing Downstream processing of metabolites is a multistage operation and broadly divided in