THIN LAYER CHROMATOGRAPHY: A TOOL OF BIOTECHNOLOGY FOR ISOLATION OF BIOACTIVE COMPOUNDS FROM MEDICINAL PLANTS

This is an abstract of a review article, is accepted for publication in next issue of 2013 in International Journal of Pharmaceutical Sciences Review and Research having Impact factor 1.336 with Index copernicus value: 5.19 (ICV 2011: 5.19) also indexed in Directory of Open Access Journal (DOAJ), Sweden; Index Copernicus International Ltd, Poland; Urlich's Periodicals Directory, Proquest, UK; EBSCO Publishing Inc (Academic Search Complete), USA; NewJour-Georgetown University Library, USA; Dayang journal system, Korea; Pharmpedia, Canada; Chemical Abstracts Service (CAS), USA and SOCOLAR, China

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THIN LAYER CHROMATOGRAPHY: A TOOL OF BIOTECHNOLOGY FOR ISOLATION OF BIOACTIVE COMPOUNDS FROM MEDICINAL PLANTS

Sanjeet Kumar^*, ¹    K. Jyotirmayee² and Monalisa Sarangi²

1.        Department of Botany, Ravenshaw University, Cuttack - 75 3003, Odisha, India

2.        MITS School of Biotechnology, Bhubaneswar -75 1024, Odisha, India

*Corresponding author’s E-mail: sanjeet.biotech@gmail.com

ABSTRACT

In this study, an attempt was made to review the basic principles and the importance of Thin Layer Chromatography (TLC) in research in general and in phytochemistry in particular. Thin layer chromatography is a simple, cost-effective, and easy-to-operate planar chromatographic technique which has been used in general chemistry laboratories for several decades to routinely separate chemical and biochemical compounds. Traditionally, chemical and optical methods are employed to visualize the analyte spots on the TLC plate. Also it has a wide application in identifying impurities in a compound. Study highlights the review on TLC and its application of qualitative and quantitative estimation of bio-active compounds from medicinal plants.

Keywords: Thin Layer Chromatography, Principle of TLC, Advantages of TLC, Applications of TLC.

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