Comparative antibacterial studies of in vivo and in vitro leaves of Lawsonia inermis L.- A multipurpose medicinal plant
Plant Science Research 35 (1&2) : 47-50, 2014 A. Moharana1, S. Kumar1, P. K. Jena1, S. K. Naik1, S. Bal2 and D. P. Barik1Ψ 1 Department of Botany, Ravenshaw University, Cuttack-753 003, Odisha, India 2 Directroate of Oil Seed Research, Hyderabad-500 030, TG, India Abstract A comparative antibacterial study of in vivo and in vitro leaf extracts was performed against four different bacterial strains viz., Streptococcus pyogenes, Streptococcus mutans, Shigella flexnerii and Salmonella enteric-typhii. Both types of leaf materials (in vivo and in vitro) of L. inermis screened for four different solvent systems, showed anti-bacterial activity (MIC values) against all human pathogenic bacteria tested. Of the four bacterial strains evaluated, the best MIC value (200 μg/ml) was observed in in vitro leaf extract of all the solvent system against Streptococcus pyogenes. Acetone and n-hexane in vitro leaf extract was found to be more effective (MIC = 200 μg/ml) than in vivo leaf extrac